Vacuum blood collection sequence, which one will be the first?

With human vacuum blood collection technology development, China has made great progress in the manufacture and application of vacuum blood collection tubes. Many unique advantages are obvious compared to traditional blood collection techniques. But do you really understand the standard blood collection sequence?

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The material of the vacuum blood collection tube: Glass tube and PET tube

Due to a large pH of glass tube, it is easy to cause hemolysis. While the glass tube produced by the drawing tube process with densely covered linear groove, the changeable situation all easy to cause cell walling.

Plastic vacuum blood tube is made of PET, PET tube has following advantages: light quality, easy to transport; small chance of tube wall breakage, specimen in transportation, centrifuge and test process; Besides above advantages, PET tubes also can be direct high pressure sterilized or incinerated. Good PET tube inner wall is specially processed in injection molding, which can effectively reduce the adhesion of blood cells to the tube wall and reduce the production of fibrin filaments.

Additive

Additives include: anticoagulant, coagulant, buffer, protective agent, inner wall treatment agent, nozzle treatment agent, separating glue and so on. Its variety, performance and concentration directly affect the character and test results of blood samples.

The commonly used are as follows:

01 Biochemical Type

No additive tube (Red cap): mainly used in serum biochemical (liver, kidney, myocardial enzymes, amylase, etc.), electrolytes (serum potassium, sodium, chlorine, calcium, phosphorus, etc.), thyroid function, drug detection, AIDS detection, tumor markers, serum immunology.

Clot Activator Tube (Orange red cap): commonly used in emergency biochemistry.

Serum Separation tube (Yellow cap ): mainly used in serum biochemistry (liver function, kidney function, myocardial enzyme, amylase, etc.), electrolytes (serum potassium, sodium, chlorine, calcium, phosphorus, etc.), thyroid function, drug detection, AIDS detection, tumor markers, PCR, TORCH, serum immunological detection and so on.

02 Anticoagulant Type

Heparin tube (Green cap): it is suitable for erythrocyte osmotic fragility test, blood gas, plasma permeability, hematocrit, erythrocyte sedimentation rate and general biochemical determination.

EDTAK2/EDTAK3tube (Purple cap): it is used for general hematology (blood routine examination) and blood ammonia test. It can not be used for blood coagulation and microelement examination.

Coagulation tube (Blue cap): used for fibrinolytic system (prothrombin time, thrombin time, activated partial thrombin time, fibrinogen).

ESR tube (Black cap): commonly used for blood sedimentation detection.

Glucose tube (Grey cap): widely used in blood sugar, glucose tolerance, red blood cell electrophoresis, anti alkali hemoglobin, sugar hemolysis and other items of examination.

Vacuum Blood collection sequence:

The purpose of standardized blood collection sequence is to ensure the quality of specimen collection and reduce the interaction between different test tubes. According to the regulations and experience of the standard WS/T225-2002 “collection and treatment of the blood specimen of clinical chemical test”, it is suggested that the vacuum blood collection sequence is as follows:

01 Blood collection needle sequence

Blood culture bottle (anaerobic priority)

No additive tube (red, golden yellow tube)

Coagulation tube (blue)

Other anticoagulant tubes (black- green – Purple – Grey)

02 Syringe blood collection sequence

In order to avoid the prolonged residence time of blood in the syringe and cause the blood to agglutinate slightly, the syringe blood collection sequence changed a little.

Blood culture bottle (anaerobic priority)

Coagulation tube (blue)

Other anticoagulant tubes (black- green – Purple – Grey)

No additive tube (red, golden yellow tube)

03 Attention:

A. After injection of blood, gently shake to prevent hemolysis.

B. The volume of blood injected into should be strictly measured according to the capacity of the test tube.

C. Can not pour blood from one color tube into the other color tube, otherwise it will lead to erroneous results.

D. Needles must not touch the tube wall so as not to contact anticoagulants, resulting in erroneous results.

After that, do you know the standard blood collection sequence?